This thesis examines business communication, stress in everyday life and work stress, with special emphasis on the impact of stress on business communication. Business communication is the transfer of information within the business world. The priority of a businessman is to communicate professionally on every level – inside and outside of a company, to provide understanding in the team and to improve the business results. Skillful communication means business success, and it is especially important in the areas of management, sales, customer relations, customer interaction and teamwork. In today's hectic pace of life, stress is an inevitable element of man's private and business activities. Workplace stress can be defined as a series of adverse reactions to the individual in which job demands are not in line with a person's abilities, capabilities and needs. Stressors that occur in the business environment are numerous and affect the work capacity of each individual and can lead to burnout syndrome at work which is the most adverse consequence of long-term exposure to work stress. Mobbing is also a complex problem of moral abuse at work that has consequences on the victim's mental and physical health. This thesis emphasizes the need for a healthy work environment and education with work stress prevention measures as an imperative of every organization. It is well known that dealing with people is extremely complex and is a significant source of stress. This thesis also emphasizes importance of the assertive style of communication and the connection of stress in business communication with the organizational structure and the role that man has in the organization have been. Very often the wrong way of communication leads to high intensity of stress and also to conflict which is stress in itself. For the purpose of this thesis, research was conducted on employees of marketing agencies about work stressors and stress in communication, as a great source of stress. The study involved 196 respondents, employees of 15 Croatian marketing agencies. The results of the study showed that although the intensity of stress in daily communication is high, the three most rated stressors are inadequate remuneration, long hours and scarce support from superiors. The study also found that women experience higher levels of communication stress than men, as do those with more senior experience than those with less seniority.