Management functions are an essential element of effective management of any organization, including organizations in the field of culture. This paper focuses in particular on the application of management functions in the context of museum activity, where the basic and special functions of management in the field of culture are analyzed in detail and identified, which enable managers in culture to successfully lead an organization in culture. The application of managerial functions in the business of an organization in culture is shown on the example of the Vučedol Culture Museum. A case study was made and research was conducted using the in-depth interview method with the aim of researching how managers use management functions in practice to achieve successful management of a museum organization and whether the application of functions allows achieving optimal results in museum work. The paper shows the importance of the application of management and managerial functions in the context of the successful alignment of management theory and the practice of management functions. The application of management functions has a key role in strengthening the organization from within and improving its competitiveness on the market. The successful alignment of management theory and practice of management functions contributes to the harmonious functioning of the museum organization. Organizations that consciously apply management and management functions tend to achieve better results and create advantages compared to those organizations that do not adhere to these management principles and practices.